Dose anyone use Sigma Ammonia assay kit?

More Kapil Singh Narayan's questions See All

What is the effect of muriate of potash (KCl) in growing chilli and tomatoes?

I want to know the effect of KCl fertilizer application on chilli and tomato farm.

20 June 2024 3,486 2 View

How to calculate dosing of polyherbal formulation and what quantity of individual herbs should be taken to prepare PHF?

How to calculate dosing of polyherbal formulation and what quantity of individual herbs should be taken to prepare PHF.

02 May 2024 2,796 0 View

How to treat green mold contamination in mushroom substrate?

I have conducted a mushroom cultivation trial for study purposes. I inoculated my substrate with oyster mushroom spawn about a month ago. Now, it has completed the incubation period. There is a...

21 January 2024 5,359 4 View

Does the carbon nanotube content the light to get inside it?

Along with scientific studies, your scientific thoughts are highly welcomed.

03 November 2023 9,571 2 View

I want to measure the work function of fused silica. Is it possible to measure the work function using UPS? Does anyone have experience?

I want to measure the work function of fused silica. Is it possible to measure the work function using UPS? I have seen several papers using UPS, but did not get the concept behind that. Does...

02 November 2023 3,779 4 View

Why Oleic acid model treated 24 hour FBS free DMEM for induce NAFLD ?

Most of paper refer this model to induce NAFLD however, why most of method followed DMEM without FBS to initiate NAFLD

01 November 2023 3,510 0 View

How do i find out top cell filtered spectrum for bottom part of the tandem perovskite/Si solar cell?

I want to calculate filtered spectrum.

29 October 2023 142 0 View

How do we make chemical structure of amorphous carbon tube for computational study ?

I need to make an input file for quantum espresso.

16 October 2023 1,232 3 View

Can anyone explain how to solve the SDDDE with implicitly defined delay?

MATLAB command 'ddesd' is use to solve the SDDDE equation when the delay is defined explicitly. However, I don't understand how to use 'ddesd' command to solve SDDDE equation when the delay is...

05 October 2023 2,185 0 View

What is the Equivalent Energy (MJ) of Elemental Sulphur?

What is the equivalent energy of the element Sulphur? with appropriate reference. I need to calculate the energy input from Sulphur fertilizers. Thanks in advance.

05 August 2023 4,731 2 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

Is it possible to improve the reforming section of the Haber-Bosch process without using air separation units?

A typical reforming section in a large-scale Haber-Bosch process (Figure 1) comprises two reactors. A mixture of methane (CH4) and steam (H2O) is fed into a primary reformer to produce syngas via...

01 August 2024 7,051 5 View

What Are the Best Alternatives to the MTT Assay for Assessing Cell Toxicity of Colored Herbal Extracts?

I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...

31 July 2024 193 4 View