How I can create neural network model for simultaneous determination of mixture of two drugs?

I want to help me in creating neural network for simultaneous determination of two drugs. I make 26 mixture of the two drugs this is my inputs data, the spectrum of the mixture of the two drugs...

19 May 2020 10,005 3 View

Why the High Voltage DC (HVDC) electric power transmission lines become to widespread?

01 May 2020 5,017 15 View

I want to use principle component analysis using matlab to simultaneous spectrophotometric determination of two drugs ?

The attached file is matlab file contains matrix (A) for the absorbance of the two compounds, the first 9 column is for compound x and the last 10 column is for compound y matrix C for...

13 April 2020 5,031 2 View

Is there any measurement of Task Based Language Teaching (TBLT) perception?

Perception of TBLT is significant because perception changes the attitude and readiness to use something. But how to measue the perception of TBLT among high school English teacher.

24 February 2020 6,239 15 View

Is there a Matlab function that solves a system of non-linear equations better than fsolve ?

I need to know if there is a Matlab function that solves a system of non-linear equations. I used fsolve but it gives some errors which are not accepted for me. I tried lots of initial values for...

11 October 2019 5,380 5 View

Which books would you suggest me for reading on 'Content Analysis'?

I want to know much about content analysis for writing methodology section of a research. please suggest me some books' name.

02 March 2019 127 6 View

How to solve three non-linear equations in MATLAB using Newton Raphson Method?

I tried to develop a code in MATLAB to solve 3 nonlinear equations using newton raphson method, but it seems that the code is not working, does anyone have the ability to fix it: function...

01 November 2018 2,976 5 View

Can I used conventional pcr to detemine microRNa rather rather than real time pcr to diagnosis breast cancer?

Pcr rather than real time pcr to detemine microRNa

07 April 2018 2,322 3 View

Super-competent cells and transformation protocol for anaerobic bacteria

I don't know, if competent cells and transformation protocols used for E. coli also applied to anaerobic bacteria such as Clostridium acetobutylicum?

22 June 2017 7,431 2 View

In some genes, Why the protein expression higher than mRNA expression and in some types visa versa ?

In some genes, why the protein expression is highly than mRNA expression and in some types visa versa?

18 January 2017 1,932 1 View

Unable to detect a truncated protein variant, but I can observe a truncated cDNA from Sanger?

Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...

17 February 2021 1,130 6 View

Help needed: How to map AA, AG, and GG genotypes on to polymorphism information?

Dear Colleagues, I am new to genes and genotyping and I need help. I am trying to figure our how AA, AG, and GG genotypes map on to A1 for DRD2/ANKK1 Taq1A polymorphism(rs1800497) and Val or MET...

02 February 2021 3,861 3 View

Using the Taqman PCR, how does one know which allele is being determined by VIC / FAM?

I am exploring a number of SNPs, unfortunately although the test are ok, I am perplexed because my preliminary results are nearly all against what is reported in the literature. To confirm my...

29 January 2021 3,905 2 View

Problems with allele speration by cloning in pKS plasmid?

I am trying to do the allelic separation of a ~8 kb region using cloning strategy with pKS plasmid. However, when I analyze the result of the colony PCR sequencing It seems that both alleles were...

14 December 2020 5,829 2 View

Weak SSR peak on capillary electrophoresis trace - is it a heterozygote?

Looking at the attached image, the top two samples show a weak peak at 212 compared to the very strong 200 peak. The bottom samples is homozygous for the 212 peak. Since the samples look a bit...

27 October 2020 2,353 3 View

How can I differentiate the true allele from stutters and PCR artifacts in microsatellite genotyping?

Most of my microsatellite markers are dinucleotides, therefore they show stuttering. In regular stuttering is easy to identify the allele, which is the highest peak on the right, after the shorter...

26 September 2020 1,486 5 View

Hi for every one I need the primer for ABO rs495828 ( ARMS or allele specific PCR)?

the primer for PCR ( ARMS or allele-specific ) for scientific research either from the studies or someone who has the ability to deisgn the primer for me as a required features above/ thank a lot...

20 September 2020 224 4 View

Is there any way to design allele specific PCR primers for a single nucleotide "deletion"?

Hi, I am currently doing a research on Inborn errors of metabolism in Sri Lanka and for that, I need to develop a method to identify few SNPs and a single point deletion using allele specific PCR...

27 August 2020 2,411 4 View

Does anyone know how can I design primer for hla_DRB1*13:03 allele?

I would like to investigate association between this allele and a disease, but sequence of this allele is very similar with other alleles in hla_drb1 alleles group, I don't know how can I design...

18 August 2020 6,560 2 View

How to test if two SNPs within a gene are on separate alleles?

Hi everyone, I have a question regarding sequencing SNPs. I have currently creating two SNPs on one gene and these SNPs are about 8kb apart from each other on two different exons. I would like to...

04 August 2020 5,531 3 View