I was wondering if it was possible to put the samples through a short centrifugation, here is the protocol:
1: Add either “UTM” or “Water RNasi-free” to nasopharyngeal swabs.
2: Vortex.
3: Transfer the sample to an RNasi-free tube.
4: Centrifugation; 12,000rpm for 1 minute at 4°c.
Sometimes the sample is full of mucus and it is difficult to take the sample to dispense it into the plate that will go to the automatic extractor.
I was wondering if it was possible after vortexing to put the samples through a short centrifugation; 4°c for 1 minute at 12,000 rpm (as reported in step 4) and collect the supernatant.
Nucleic acids should remain in the supernatant.
Thank you in advance for your reply.
What we do in our lab is that Nasopharyngeal/throat swabs mixed together in 3 ml UTM. Then each 1000μl of the sample will be centrifuged at 10000g/min for 5 min, then discarded the supernatant except 100 µl of the solution will be leaved to be used in re-suspension of the pellet for RNA extraction (since the viruses are present intracellular).
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