Instead of FBS or FCS. If I use horse serum in cell culture, will it lead to activation of complement system and death of cells?
Please try with horse serum and find out and let us know from your experiment, which will definitely say an answer. good luck. lalitha kabilan
Often, horse serum is used to stop proliferation of cells, without inducing cell death. As example, I use horse serum to stop proliferation of myoblast and induce fusion in myotube.
you have to heat it (56ºC, 30 min) before use it for cell culture. We use it for cell culture.
We use horse serum in combination with FBS for culturing myoblasts and it works pretty well.
Just be sure you don’t have a lot of debris in your serum, it will lead to cell dead.
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