5% tween 80 in CMC showed cholesterol lowering in DIO mice after 7 day treatment. What will be the possible mechanism?
I have data in the form of intensity versus kinetic energy. I need to convert kinetic energy to binding energy. Ultimately, I need to calculate the work function and the position of valence band...
11 February 2024 8,741 1 View
difference between excitation temperature and electron temperature in plasma
09 January 2024 3,078 2 View
what is excitation temperature of electron in plasma
09 January 2024 9,676 2 View
Please give complete recepie and preparation protocol
22 December 2023 3,043 0 View
I have a system of DNA and a certain molecule that shows good docking energy with DNA. I want to run the simulation in gromacs. The parameters I should use for DNA is amberff99parmbsc so I tried...
19 October 2023 2,152 2 View
For the optimization of your emulsion sample, how one should prepare the sample to make a ternary phase diagram ? Please mention the ratio and method of preparation. Thank You.
02 July 2023 2,310 1 View
surfactant for emulsion
28 June 2023 8,994 2 View
Hi ResearchGate community, I am interested in using chemical flocculation to concentrate water samples for eDNA analysis. This is basically to avoid having to filter water. I have come across...
13 June 2023 7,552 1 View
Hello, I am interested in understanding the relationship between binding energy (B.E.) and ions' oxidation state (O.S.) in XPS spectra. Per example The core-level XPS of Mn2P in MnxNy can be...
21 December 2022 1,710 2 View
Suppose a particle mass is m=m_{0}[ 1+ i\beta x] where \beta
10 February 2022 2,556 25 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View