Our group has noticed a fairly high test fail rate when using the Charles River Endosafe PTS system after performing endotoxin removal via Triton X-114 phase separation. Normally this process does leave behind a small amount of detergent. So, can the small amount of detergent interfere with endotoxin quantification by the Limulus amebocyte lysate test?
we routinely use Charles River instrument and assays. It is the most expensive option (30-40$/test) but its FDA approved, very easy to use, reliable and accurate. Many other kits and test that we tested were suboptimal (Lonza etc). That being said, even Charles River assay sometimes needs some optimization for the new samples. You definitely need to find the best dilution, depending on what is present in your sample- plasmid DNA, salts, etc. Mg and other additives might be helpful. Not sure about Triton, might be interfering. Definitely do your best at removing it from the sample, and dilute your plasmid 250-1000x for readout
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