I would like to request researchgate people to comment on the following abstract. Does this report any findings, or convey a message? What are the weakness of this abstract?
Improved design of a synthetic Bt gene stack and testing its insecticidal efficacy in the model plant Arabidopsis
Bacillus thuringiensis (Bt) insecticidal toxin protein encoded by Cry gene is a widely used technology to control insect pest in the crop field. However, development of insect resistant to Cry genes has appeared as a major threat to the durability of this approach, and thus urgent action is required to overcome this problem. Out of many available approaches, stacking of multiple Cry genes in the same plant is thought as the best strategy to delay the development of insect resistant to Cry genes. Here we report the insecticidal activity of a genetically engineered Bt gene stack consisting of Cry1B/Cry1C genes in the model plant Arabidopsis. Cry1B/Cry1C genes were designed to produce a novel version which is free of IP. Components which have the freedom to operate were used to test the insecticidal activity of the modified Cry1B/Cry1C gene stack. Availability of technology that does not require licensing agreement to use, is one of the main barrier to develop GM crops by public sector organizations in the developing countries. Thus, it is expected that the modified Cry1B/Cry1C gene stack will be a valuable tool to develop GM crops for public or humanitarian use in the developing nations.
This abstract doesn't give any finding. it gives only suggestion about new methodology about gene transfering. the abstract needs key points about new methodology (why we are prefare, what will be happen when we use more than two Cry genes etc.)
it is still interesting subject. the review will get more attention.
I too suggest that you need to sum up the major findings of your study and show whether the findings back up your hypotheses.
The Abstract already gives the goal, and the approaches of this study. It would be nice if a few major results/discoveries are included at the end.
This is novel research. I RECOMMEND THE AUTHORS.
As asked by one the authors whether the abstract report any finding or not, in fact, no finding/discovery is reported in this abstract, but the gold is well stated.
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