Md. Mahmudul Hassan

19 Questions 11 Answers 0 Followers

Questions related from Md. Mahmudul Hassan

How to assemble four gblocks into a vector using Gibson assembly?

Hi All, I am trying to generate a bacterial operon construct. I have designed four gBlocks (each of which is ~1.7 kb) and synthesized them from IDT. I am using pGex-6p-1 vector to assemble these...

07 July 2019 8,434 4 View

Where to find pML-BART, pART7 and pART27 vector?

Hi All, I am looking for several plant expression vectors (pML-BART, pART27 and pART7). Could anyone please tell me where I can find these vectors? I have searched in google but did not get any...

07 July 2019 1,810 10 View

How to make operon construct for homologous recombination mediated gene integration in E. coli chromosome?

I am planning an experiment where I will integrate an operon construct in the gfc locus E. coli using CRISPR-cas9 mediated homologous recombination. To insert my operon construct in the gfc locus,...

05 May 2019 9,259 3 View

Can anyone please help me to explain the following qRT-PCR result?

I generated several transgenic Arabidopsis lines that contain a T-DNA in which two transgenes (Gene A and Gene B: see attached figure for more details) are linked. Each transgene has its own...

01 January 2018 5,226 13 View

Could anyone explain why some Arabidopsis genes are present as single copy in Brassica napus genome?

I am trying to identify and elucidate the function of a gene (let say X) in Brassica napus that control flower development in Arabidopsis. Using the sequence of Arabidopsis X, I conducted a...

12 December 2017 1,626 3 View

Does this abstract report any findings?

I would like to request researchgate people to comment on the following abstract. Does this report any findings, or convey a message? What are the weakness of this abstract? Improved design of...

12 December 2017 1,161 5 View

What is the function of sucrose in plant transformation solution used for floral dipping?

Presence of sucrose and surfactant in transformation media are the critical factors that affect the efficiency of floral dip transformation. Can anyone explain how sucrose help in transformation...

08 August 2017 5,987 6 View

Faint band in negative control in plant genotyping PCR?

I am genotyping transgenic plants for the presence of transgene which is an artificial miRNA construct. I am using miRNA specific primers to genotype the putative transgenic plants which I...

02 February 2017 8,354 13 View

Where is the problem in my cloning?

I posted this problem before and Now I am posting it again as I am still having the same problem. Please see and help me to find the solution. "I am trying to clone a 7.3 kb insert into a plant...

07 July 2016 1,252 26 View

Which is the best E. coli strain for unstable plasmid??

I am trying to make a plasmid by traditional cloning. The size of the insert is 7.3 kb and the size of the vector is about 7.2 kb. I am using PacI to isolate insert and prepare the vector. I had a...

07 July 2016 2,293 5 View

How can I identify the number of genes from cloned cDNA sequence?

I am trying to determine the copy number of a gene which control flower development in B napus. I have done the following works to do that. 1. Isolated mRNA from from B napus flower bud and...

01 January 2016 2,180 7 View

Which is the best method for identification of transgene insertion sites in the plant genome?

I have generated several transgenic Arabidopsis lines by Agrobacterium mediated transformation. Now, I want to map the genomic position of T-DNA in these lines. What will be the best and easiest...

01 January 2015 3,267 12 View

How can I design a probe for southern blotting?

Hi All I am planning to do southern blotting in order to detect the transgene copy number in plants. Can anyone please suggest me how can I design my prob? Also, which method of detection,...

01 January 2015 6,969 9 View

What will be the size of breeding population to combine two transgenes by crossing?

Dear all I know that genetically unlinked transgenic locus will segregate in the progeny and therefore need to screen many progeny population in order to find out the right combination of gene...

12 December 2014 7,545 7 View

How to clone very large insert into large vector?

I am generating a targeting construct for homologous recombination in plants. After several subcloning rounds, the size of the insert is now fairly big; one is 6650 bp and the other is 6498 bp....

03 March 2014 1,182 10 View

Is there too much background colony in the control plate?

Over the last few months I have had serious problems in cloning as a result of too much colony in control plate. Here I am explaining what I did: I was trying to clone a 1.2 kb and 3.4 kb insert...

03 March 2014 2,879 10 View

Anyone have experience with cloning a 5.1 kb insert into a 13.5 kb plant binary vector using Sacll site?

I am trying to clone a 5.1 kb insert into a 13.5 kb vector ( plant binary vector) using SacII site. My recipient vector is a plant binary vector (pPIPRA560) and my experimental procedures were as...

02 February 2014 10,048 1 View

Problems with Cloning Bt cassette?

I am having problems with cloning 2100 bp fragment which contains selectable marker gene bar (herbicide resistant) with lox site flanking the bar fragment into a vector nearly 13kb. I want to...

09 September 2013 236 2 View

What is correction factor in ANOVA analysis? Why is it needed during ANOVA analysis?

I have found in a book ( Statistical Procedure in Agricultural Research by Gomen and Gomez), the authors used correction factor to estimate total sum of squares. I am interested to know what this...

01 January 1970 9,607 2 View