I have an Sf9 cell culture in a T75 bottle. After 3 days I observed that many cells were strongly adhered in the bottle inner surface. In the day 3 I observed some suspicious particles that I thought were bacteria. Thus, I supplemented the culture media with anti-anti (antibiotic-fungicide). When today I observed the bottles, all the cells were in suspension. Was the excesive culture time or was the anti-anti?

Thanks in advance

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