I have tried different protocols for nano particle synthesizing of plant extract using copper acetate, still i could't obtain a positive result. As my supervisor instructed not to heat CU.Act.Nps + Plant extract at above 50 C temperature for a long time because it might degrade the important phytochemicals in the plant extract. I have used lastly 1mM, 90ml of CU.Act solution 20 mins of stirring mixed with 50 C Distilled water, then added with 10ml of plant extract followed by 20 mins of stirring, finally 24 hrs Dark incubation. Please provide me a best protocol to obtain a good CU.Act.Nps...............