What is the best antimicrobial agent among metal oxides?

I am looking for an antimicrobial metal oxide agent for a polymeric implant and I am wondering which one is the best?

21 January 2021 7,068 5 View

Detection of insect eggs in stored and processed products?

Currently, in order to identify insect eggs in stored or processed products (such as contaminated flour or wafer samples), staining was performed based on the method mentioned in the below...

15 January 2021 6,362 5 View

Why pathlines keep changing their patterns?

Hello, I am using ansys fluent to simulate a steady state phenomenon, even though the residual curves are absolutely flatten, pathlines keep changing their patterns over more iteration. I was...

12 November 2020 2,231 3 View

How to simulate fracture in the axle housing ?

Hey there, I want to simulate & analyze the fracture and fatigue (crack growth) in the rear axle housing of heavy tracks (Volvo company) in Abaqus. My question is which method is suitable for...

12 November 2020 3,377 3 View

Do you think Corona-virus (COVID-19) will lead to a new age of environmental behaviors?

It seem individual's attitude to environment are changing rather than last year due to corona virus issues and challenges. What do you think?

27 August 2020 6,394 17 View

Which algorithm (Sobel, Perwitt, Roberts, Canny and LOG algorithm) is more capable of finding noisy image edges?

The edge detection algorithms are designed to respond to sharp edges which can be caused by noisy pixels. In image analysis edge detection plays an important role and it is one of the traditional...

05 August 2020 2,543 1 View

How can I request a transimtted force between two nodes when I use tie constraint in ABAQUS?

Dear all, I have used a tie constraint in ABAQUS to attach two surfaces. the meshes of both surfaces are compatible so in front of every node of surface-1 is a node of surface-2. thus the force...

24 May 2020 9,890 3 View

What is the best way of Evaluation Performance for Multiclass Data?

For classification problems, classifier performance is typically defined according to the confusion matrix associated with the classifier. Based on the entries of the matrix, it is possible to...

19 May 2020 3,472 4 View

Best EEG dataset for sleep stage classification?

I am looking for a best dataset with signals of EEG that is used for sleep stage classification.

12 May 2020 5,935 3 View

What are the public biosignals (ECG, etc) datasets of COVID-19 patients?

I am researching QOVI-19 and need a database that contains biosignal data such as electrocardiograms. Is there any dataset?

12 May 2020 9,274 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View