Hello,
I want to know if anyone has been able to get large RNA fragments from performing RNA extraction through the RNeasy mini kit.
Many thanks!
Jeroen
Hi Jeroen,
yes, you should not have any problem, since all RNA molecules larger than 200 nt are purified with this kit (see page 8 of the Handbook):
https://www.qiagen.com/us/resources/resourcedetail?id=14e7cf6e-521a-4cf7-8cbc-bf9f6fa33e24&lang=en
Thank you for your reply, I was mainly concerned about rapid degradation of these large fragments.
I agree with the rest of the replies. If you are concerned about the degradation, just be sure that you add RNAse free reagents, apart from those supplied by the kit (eg: ethanol).
Plus, carrying out the purification in a laminar flow hood will extremely reduce RNA degradation.
Nevertheless, I don't think large fragments tend to degrade more easily than the short ones
We have successfully used this kit to re-purify CTV RNA extracted by TRI method. CTV is almost 19Kb RNA genome.
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