How do I remove excess Polyhydroxymethylsiloxane from the final product?

I did a simple organic reaction where I left with excess of Polyhydroxymethylsiloxane (PHMS) in the final product solution. I tried to extract it with aqueous layer, but I failed. Let me know, if...

08 September 2015 2,705 3 View

Is there a visualizer which could show amino acids involved in Protein-Protein interaction?

06 July 2015 9,678 15 View

What will be the effect on RMSD during to superposition of proteins?

when we do superpose of 3 pdbs together (1 on 2, 2 on 3, 3 on 1) and after that if we change the sequence of superposition, will there be any possibility to see the change in RMSD value (before...

05 June 2015 8,043 7 View

How can I dock a ligand in more than 1 receptor?

I have 6 receptors and only 1 ligand. In the meantime I interested to do the docking in all these receptor. Is there any way where we have a generalised way to dock this compound to these receptor...

10 November 2014 4,379 12 View

What is the best scoring function/force field for docking carbohydrates ?

10 November 2014 3,066 5 View

Molecular dynamics of Protein-small molecule for Windows?

Hi I am looking for a MD simulation calculations for Small molecule-Protein interaction. But right now I am limited to Windows. So please let me know if there is any software which is useful in...

10 November 2014 3,788 10 View

Is there any free software available for inverse docking or docking, where we are searching for a receptor or protein for a known ligand?

I want to know about the tools available for searching for a protein or receptor for a known ligand.

09 October 2014 6,140 9 View

How does Dinitrobenzaldehe reduction happen to Diaminobenzaldehyde?

What is the best reagent which can be useful in the reduction of 2,4-dinitrobenzaldeyde?

07 August 2014 1,744 16 View

Do we have any method to find out acetylation on any protein?

What we know is that the proteins get acetylated at lysine moiety. But looking into the structure, we got around more than one lysine moiety. Then how it can be possible to find out which lysine...

07 August 2014 1,090 6 View

What method is employed to check the structure/molecule stability?

Recently I was encountered with a problem where structure shown in figure is not able to get converted to any product. May be possibly due to its resonance effect or any other effect between the...

06 July 2014 1,563 18 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Do I need to introduce HFD to ob/ob mice to obtain a T2DM model?

I am planning to work on T2 diabetic animals and check the efficacy of probiotics. My initial plan is to fed B6 animals with HFD for a while. But now, I have the chance to work on ob/ob mice. My...

18 February 2021 6,896 3 View

Can anyone suggest a way to dissolve insulin which is for rat/mouse injection?

We are trying to inject insulin to rat or mouse, but the insulin we've got is powder, and there are many ways for dissolve, like in saline, PBS, DMSO, or in Tris-HCl, can anyone give us a...

13 February 2021 8,426 10 View

Bradford assay for insulin: discrepancies between standard curve and sample concentration during crystallisation?

Hi there, I would like to determine the unknown concentration of human insulin in my sample during crystallisation (the concentration range is roughly 0.5 -2 mg/ml, whereas the initial...

06 February 2021 9,370 7 View

Is there a protocol for the insulin encapsulation at physiological pH?

Hello everyone, I am going to start my master thesis on the encapsulation of insulin. In almost all protocols we found an acidic pH is necessary. Unfortunately, pH

27 January 2021 3,692 5 View

How seniors face covid 19 without fear?

Seniors in the world with diabetics and hypertension and heart diseases are millions ,and they are facing covid.therefore we should give them advices and precausions to face covid 19,this...

26 January 2021 8,349 4 View

About protocols used for glucose uptake in HepG2 cells?

Hello everyone. I am curently working on a protocol to measure glucose uptake in HepG2 cells using 2-NDBG. The work involves the pre-treatment of cells with an active material and observe if it...

25 January 2021 7,608 3 View

What is role of chronopharmacology or circadian rhythms in metabolic diseases including obesity, diabetes and cardiovascular diseases?

Chronopharmacology is branch of biological science which deals with daily life, sleep and wake, day and night, feeding and fasting and other effects. we can manage many diseases including...

18 January 2021 9,045 3 View

What type of interventional study is this?

Hi, I'm evaluating some medical papers to write a review, but I'm having trouble defining the study design of the following paper: Neurophysiological recovery after carpal tunnel release in...

04 January 2021 1,571 4 View