Our main goal is to determine if the dosage of a certain drug increases the post-operative appetite of dogs.

Some key points worth mentioning about the research are the following :

  • In healthy dogs this certain drug increases the appetite.

  • (Phase 1) 6 hours after the operation we provide dogs with food and if they do not eat we provide the drug + food.

  • (Phase 2) 12 hours later we provide the remaining dogs (those who did not eat in Phase 1) with food and if they do not eat we provide the drug + food.

In the pdf attached I have drawn a graphic representation of the study.

What statistical model could be implemented and what is the minimum sample size in such cases? Any bibliography suggestions would be really welcome.

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