In a Western Blot (WB) experiment, does the concentration of SDS-PAGE gel affect the position of protein bands? In the image, the same protein samples were used for all five lanes. The left side (lane 1, lane 2, lane 3) used a 10% gel, while the right side used a 12.5% gel, with all other conditions being consistent.
Thanks for your kindly help!
No concentration should not affect the position. However it significantly affects transfer. You are likely to have less signal for upper band than lower band if you increase concentration the gel since it is difficult for big proteins to be transferred from higher concentration gel
Hi Quan,
The concentration does not play a role in the change in banding pattern. You might observe thicker bands, but that is pretty much all that you should see a difference in. From your image, I still see the same protein bands at the correct size in both the gels, only the intensity varies. This should not be a problem :)
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