Quan Yang

6 Questions 2 Answers 0 Followers

Questions related from Quan Yang

The differences between glycine buffer solutions of the same pH but different concentrations lie in several aspects?

What are the differences between 50 mM glycine-hydrochloric acid buffer (pH 3) and 100 mM glycine-hydrochloric acid buffer (pH 3), especially regarding osmotic pressure? Why do they have the same...

25 March 2024 8,879 1 View

Acid stripping cells by washing with low pH solutions dissociates ligands from their receptors?

hello ，I want to use exogenous ligands to activate receptors on the surface of the U87 cell line. How can I remove endogenous ligands before the experiment to ensure a low baseline receptor...

14 March 2024 3,208 1 View

How to stimulate uPAR by uPA in cell alive?

According to the experimental procedures provided in several reference , I attempted to remove endogenous uPAR from the cell surface using an acidic solution. The protocol involved washing the...

13 March 2024 782 0 View

Does the concentration of SDS-PAGE gel affect the position of protein bands?

In a Western Blot (WB) experiment, does the concentration of SDS-PAGE gel affect the position of protein bands? In the image, the same protein samples were used for all five lanes. The left side...

30 January 2024 9,480 2 View

Western blot for GPI-anchored proteins ?

How to perform Western blot for GPI-anchored proteins, and are there any differences in the experimental conditions, particularly in the extraction and processing procedures of protein samples,...

29 January 2024 4,243 0 View

Western Blot for detecting uPAR ?

Dear esteemed researchers, I have recently conducted Western blot experiments to detect uPAR (CD87). The whole-cell lysate samples I used were obtained from four human glioblastoma cell lines...

21 January 2024 4,608 2 View