I think so.

It would depend on how fresh the K-acetate is, what ethoh percentage you are using and of course the DNAase you have floating around your lab.

In isolating the DNA from fecal matter i use binding buffer which contains 4M guanidine Hydrochloride and 1M potassium acetate,pH 5.5.and an equal amount is added to supernatant.

We isolate the DNA from blood samples and use only Small qut. of blood ie only 300ul .

Dear all,

I have one question, what would happen if after alkaline lysis, the potassium acetate buffer is kept for longer than 10 mins for neutralisation? Do you think, genomic DNA will also neutralise and contaminate the plasmid DNA preparation?