I am trying to extract RNA using trizol from very small parts of honeybees e.g. antennae and mouth-parts and was taking as much of the aqueous phase as possible in order to maximise yield. Following multiple problems with DNase treatments not working we decided that phenol carry over was to blame and are now trying to find a middle ground between decent quality (260/280 ~1.8-2 and 260/230 ~ 1.8-2) and decent yield. I have started using glycoblue as a co-precipitant to increase the yield and whilst the 260/280 remains high the 260/230 never gets above 1.3, is this the glycoblue?
I just realized a precipitation of RNA from small zebrafish brains, and I can say it did not affect my precipitation or RNA quality
Thanks Raul, I think this was optimistic thinking in a hope that my samples were better quality than I thought...but I don't think they are.
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