Does Someone know if FITC is soluble in chloroform?

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Problems with Sybr green melting curve?

Hello, Do you have experience with this type of melting curve? Can anyone explain to me why there is a lowering of the curve? I'm using SYbr select master mix on quant studio 5 real time pcr.

21 May 2024 1,191 0 View

Noves Ricerca Group?

Good morning to all, I have received an invitation to publish a paper from Noves Ricerca Group in a Q2 Journal. They have read my paper and very quickly they have accepted it for publication. It...

20 May 2024 7,407 3 View

What type of high-quality nanopure water do you use for NTA analyses?

We recently acquired a ViewSizer3000 from Horiba, and the manufacturer recommended us to use nanopure water from XZero for the NTA analyses. Do you recommend using any other brands of NW? Which...

05 May 2024 1,976 1 View

What is the best way to concentrate the protein and remove the imidazole post Ni-NTA purification?

I've always struggled with concentrating and washing my proteins after purification. Often I lose almost everything in the process. I have used dialysis membranes followed by column filter...

16 April 2024 5,716 0 View

Orbitrap Astral Mass Spectrometer: applications?

Hi everyone, I'm a PhD student at CAST, Analytical Biochemistry and Proteomics Research Unit, University, "G. d'Annunzio" of Chieti-Pescara. We currently use the Thermo Fisher Orbitrap Fusion...

10 April 2024 9,346 0 View

Has anyone experience with a good one antibody for the western blot detection of SQSTM1 in human cells?

The old antibody has been discontinued and the currently offered are detecting a band at 62 kDa, which does not reflect the correct molecular weight (47 kDa).

17 March 2024 4,300 2 View

What can be the causes of which protein bands don't run through a SDS-PAGE gel?

I'm having a problem with SDS-gel. I cannot get the protein bands to run fully on the gel. For me it's the first time with SDS-gel. I prepared a polyacrylamide gel with 5% stackyng gel and 15%...

10 March 2024 228 2 View

Seeking Reviews for Q-TOF LCMS-9030 Mass Spectrometer?

Dear Forum Members, I hope this message finds you well. I am currently considering the purchase of a Q-TOF LCMS-9030 Mass Spectrometer for my laboratory, and I would greatly appreciate hearing...

10 March 2024 9,081 0 View

Can I use this buffer made 1 years ago and storage a 4ºC?

The buffer contains: 100mM NaCI, 0.1% Triton X-100, 300mM Sucrose, 1mM MgC2, 1mM EGTA, 10mM PIPES (pH 6.8), and 100uM PMSF

23 January 2024 4,464 1 View

Gerrymandering or how to detect discriminative electoral rule change? it?

In public life, democratic mechanisms can be misused. Fitting electoral rules to desired results is called Gerrymandering to remember a prominent politician who was an expertin such Machiavellian...

03 January 2024 2,849 0 View

What is the solvent for 8YSZ nano material?

I need to do some experiment with it in liquid form.

07 August 2024 613 3 View

What is saturation solubility of insulin in saliva?? I need it to know sink condition ?

05 August 2024 4,407 0 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

I need to dissolve stigmasterol for an animal experiment, it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent?

Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?

01 August 2024 7,504 2 View

How to explain the different interaction between soluble and insoluble polymer in water?

Hello everyone, I am try to understand the differences interaction between insoluble and soluble polymers in water with metal ions and how theit charge density (of metal ions) affect the interaction.

29 July 2024 6,350 3 View

How to remove/ replace trifluoroacetate countertop after deprotection?

After deprotection of the tert-butyl ester to generate the carboxylic acid in chloroform with TFA, my compound‘s pyridine moiety forms a TFacetate ammonium salt (small molecule drug, not a...

29 July 2024 9,753 3 View

What is the solubility of Iron (+3) in Bismuth Oxide?

Dear all: I am modifying Bismuth Tungstate with Hematite (Fe2O3), so I would like to know about how much Iron(+3) can form a solid solution in a Bismuth Oxide matrix. Best wishes Marco

29 July 2024 2,163 1 View

What does negative readings in spectrophotometer denote. Was testing TSS content of bacterial cultures?

I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...

22 July 2024 9,576 4 View

Why methanol and sulphuric acid used in the analysis of polyhydroxyalkanoates (PHA) by GC-MS?

Why methanol and sulphuric acid, used in the analysis of polyhydroxyalkanoates (PHA) methyl esters by GC-MS? Additionally, why do we typically use non-polar solvents in GC-MS?

22 July 2024 1,210 2 View

When making abraxane with Nab tech, why is 9:1 Chloroform&EtOH used as solvents?

Is it the best optimization ratio based on experiments?

22 July 2024 8,170 0 View