Hello,
I am working with M93 cells, and need to run death assay with them, looking for a good easy-to-work protocol.
My master's research is in info retrieval and text mining. I would be grateful if you could help me to select a good topic for my phd research proposal.
24 February 2021 393 14 View
I am working on a project monitoring crop field health. For this purpose, I am preparing my dataset using drone video. I have converted it into keyframes. Now I want to pre-process these aerial...
25 December 2020 7,529 5 View
I want to know about the dangling bond? by which method we can find it, does its need some special type of characterization just like XRD etc... or method i want to find dangling bond effect in...
26 October 2020 1,938 6 View
Hi, can someone explain which algorithm should I use to convert DN(Digital Number) values to radiance, because there are many different algorithm that I found, there are 3 for now (based on...
29 August 2020 1,479 2 View
During this pandemic caused by COVID_19 there has been a trend in the academics also due to the modernization by attending online Webinars and even some international conferences are being...
09 August 2020 342 17 View
Can you please erase the doubt from my mind regarding 'Pay and Publish' versus 'Free Publication,' because in both the cases money is being demanded either from the author or the reader. In the...
02 August 2020 1,536 8 View
What are the best tactics, suggestions and recommendations that one should adopt while converting a Ph.D. thesis into a Book and who can be the genuine or reputed publishers for it?
02 August 2020 5,260 14 View
I'm currently doing a study on potential hydrogen damage in superalloys. I'm wondering if carbide dissolution takes place in superalloy in the same manner as steel does with the potential...
12 July 2020 1,420 1 View
Hi everyone I need help in preparation samples for RAMAN spectroscopy, i am working in thin film field, spin coated on ITO or Si wafer. Can any one suggest RAMAN sample preparation method ?...
25 May 2020 7,050 9 View
Dear All, I got 2 weeks old paraffin blocks of tumor masses. I want to extract total RNA from them. The available reagents in my lab are: 1) Cell dissociation buffer (GIBCO#13151-014) 2) TRI...
30 March 2020 5,067 5 View
Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...
02 March 2021 2,616 1 View
I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...
01 March 2021 6,999 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...
28 February 2021 2,133 3 View
I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...
23 February 2021 7,842 1 View
I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...
23 February 2021 9,933 1 View
New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...
21 February 2021 3,278 3 View
Please suggest solubilisation of chlorobutanol in injections. Tried pH and high temperature. But the issue is of assay drop. If anyone can suggest any solution. Its a commonly used excipient for...
21 February 2021 7,397 1 View
I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...
21 February 2021 6,260 3 View
Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...
21 February 2021 6,633 3 View