I will perform staining experiments with Dil dye in parasitic nematodes. I have found it can be diluted in DMF, DMSO or ethanol. Have someone worked with DMSO to dilute the dye? Any advice?
If so, could someone please give some insights on the IPTG concentration, the length of the induction period, the temperature during induction, and the cell line that was used. The gene is cloned...
15 August 2023 831 1 View
Hello, I want to silence my gene of interest using ptdT-RNAi vector, so what I need to know is either if the amplicon must include the 5’UTR region, the ATG or both. I know that the length has to...
30 May 2023 307 1 View
I've seen some publications ask for one year before posting elsewhere. A university librarian informed me that no such posting is allowed, on any type of site, including for members only.
20 November 2022 9,144 10 View
I collected a seaweed sample from the Kenyan coast (Mkomani). I was looking for Caulerpa macroalgae. I am uncertain of the specific name of the pictured seaweed. Can you help me identify the species?
20 October 2021 7,530 7 View
Hello, I am doing a microsatellite PCR and the bands appear with a few definition, the electrophoresis conditions are the following, TBE buffer 0.5X pH 8.4 (according to Sambrook, Molecular...
29 August 2021 2,860 3 View
I am monitoring the degradation of contaminants using immobilized enzymes on carbon support. However, to complete the mass balance of the degraded reactant, I have to perform a mass balance on...
06 April 2021 6,802 2 View
I am struggling on how to calculate this. I know I need the MW, but I am not sure how these all tie together.
01 April 2021 4,795 1 View
Hello everyone, I did a transformation of E. coli DH10B using pUC18 plasmid. The bacterias did grow on MKL medium with ampicillin but the colonies showed a white/pink colour and the medium was...
08 February 2021 10,086 3 View
Hello, I am not a biology researcher, but I am working in intradisciplinary research and would like to know the answer to this question, which I have not being able to understand. This questions...
06 January 2021 4,195 4 View
I was looking for Caulerpa lentillifera earlier today in Kenyan South Coas in Kibuyuni, Kwale county. I found a different kind of sea grape. I am certain that it is of the genus Botryocladia, but...
06 September 2020 1,147 3 View
This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.
08 August 2024 3,746 4 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...
05 August 2024 3,290 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?
01 August 2024 7,504 2 View
99% pure ethanol was used for maceration also alternate methods along with the temperature and time to concentrate the extract can be specified
31 July 2024 5,113 4 View