Hi everyone,
I am currently working on establishing a co-culture system for NMJs. Unfortunately I have no experience how NMJ might look like in vitro. I performed some co-stainings with BTX and TUJ1 as well as synaptophysin, but am not sure if the co-localizations I see are true NMJs.
I hope someone can help me with this issue and share a few experience with NMJ stainings in vitro.
All the best,
Jessica