Does somebody in Europe have a colony of oriental fruit moth, Grapholita molesta?

27 May 2019 0 3K Report

Hi,

I would like to establish a laboratory colony of this moth. for this reason I am searching for somebody who could provide me with a starting population.

Thank you for your help!

Badges
Science topic

More Tim Haye's questions See All

What are the key theories relating to the nature and exercise of leadership?

Who are some key author to refer to?

18 May 2024 7,998 5 View

Sexual selection and speciation – unpublished data?

Dear Colleagues, We are conducting a meta-analysis to synthesize comparative studies investigating the relationship between proxies of sexual selection and species richness or speciation rate in...

07 April 2024 5,425 0 View

What can be the reason if electrode A has higher specific capacitance value than electrode B but electrode A has higher Rs value?

I have prepared two same materials with diffferent method and performed electrochemical testing. The electrode A which has higher specific capacitance gives higher (8 times) solution resistance...

25 March 2024 5,833 0 View

Which non-denaturing lysis buffer can comprehensively lyse THP-1 monocytes?

Hi, I am doing a pulldown in THP-1 wt lysate followed by MS-based proteomics. However I get a very low protein amount out of the cells (1 mg per 12 million cells). I use the following lysis...

14 February 2024 9,030 1 View

How to sovle the problem of protein sticking to Ni NTA/IDA columns after SUMO tag cleavage ?

Hi there. I have to use the pET28a-SUMO vector to get my target protein. However, I can‘t separate it from SUMO tag. My protein was about 15 Kda big and has a about 5 value of pI. Due to the...

09 January 2024 2,970 4 View

Is the cif file for MXene(Ti3C2Tx) available?

Is the cif file for MXene(Ti3C2Tx) available? If yes, can anyone provide me with one for performing rietveld refinement? I have search from COD and AMCSD website and can't seem to find one.

13 November 2023 5,000 2 View

Is spatial autocorrelation always a problem while modelling habitat suitability?

I'm wondering if you should differ between presence data of highly mobile species such as Raptors and immobile species (e.g. Plants). The dispersal of plants is limited to a certain distance, so...

23 May 2023 6,666 4 View

How to prevent sticking of single cancer cells to PDMS microfluidic chip?

I'm doing a flow experiment, flowing single MCF7 cells through PDMS microfluidic chips (connected to a glass microscope slide). Currently, I'm using a Pluronic F127 (0.1 wt%) coating. However,...

21 May 2023 9,628 3 View

Difference between power and myAUC in Seurat R package for analyzing Single Cell RNA-seq data?

I am attempting to use the Seurat FindAllMarkers function to validate markers for rice taken from the plantsSCRNA-db. I want to use the ROC test in order to get a good idea of how effective any of...

16 May 2023 2,909 1 View

Difference between power and myAUC in Seurat R package for analyzing Single Cell RNA-seq data?

I am attempting to use the Seurat FindAllMarkers function to validate markers for rice taken from the plantsSCRNA-db. I want to use the ROC test in order to get a good idea of how effective any of...

15 May 2023 6,047 0 View

What are the best practices for washing and preparing fruits and vegetables to reduce pesticide residues?

27 July 2024 9,147 4 View

What is the best way to drop a plasmid?

Please address the best way to drop a plasmid. Background: I have a "bait" plasmid resistant to kanamycin and a "prey" plasmid resistant to carbenicillin. After many rounds of streaking on...

25 July 2024 2,532 3 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

My rosetta strain has chloramp resistance and i am transforming my plasmid with amp resistance but not getting colonies on chloramp/amp LB plates?

I have done the same procedure with BL21 and pLysE but i got colonies but multiple times on rosetta i did but not getting. can anyone explain what would have gone wrong?

12 July 2024 9,904 1 View

Has anyone calculated the effective concentration at 50% (EC50) value of different fungicides for the fungus Tilletia horrida?

How can we measure their colony diameter?

12 July 2024 7,027 0 View

I have constucted a plasmid vector from puc19 with no antibiotic and lacz gene. The problem is screening for transformed cells. Any suggestions?

Get too many colonies, which are mostly non-transformants when tested by colony PCR

11 July 2024 6,775 3 View

I am not getting proper colonies while ligating one of fragments in my vector the colonies seems to be normal but in light it seems lysed any reason?

I am working on cloning of a gene fragment inside my vector everything we tried to do correctly like making insert and vector with utmost care but after ligation the morphology of colonies seems...

11 July 2024 9,010 3 View

Can type 2 diabetes be reversed at any age through proper diet and exercise?

Type 2 diabetes can be managed and, in some cases, put into remission through proper diet and exercise, regardless of age. Here are some key points: Weight Management: Achieving and maintaining a...

07 July 2024 8,745 2 View

What is the necessary and sufficient condition for exism movements to become permanent authoritarianism from within liberal democracies?

You have seen the comings and goings now of Trumpism, Brazilianism, and Brexism, and the common theme is why they failed to persist in power ONCE THEY CAME TO POWER. If you look at the evolution...

06 July 2024 7,050 1 View

Can someone provide any tips for cell quantification on Fiji, ImageJ?

Hi. I have brightfield images of stem cells. I need to count the total number of cells/image. Some cells are individual and some cells are in colonies. Using average cross-sectional area of...

05 July 2024 4,197 1 View