Jacob Simons I can't share the details of my experiment here I am not allowed but I am facing a problem and I suspect it's related to seed quality , I tried to find resources to support my argument but didn't find anything helpful
The way you worded your question made me think you were a lazy student.
We need more information. What is your problem? Low or poor germination?
Does the seed require light to germinate? Does the seed have a hard seedcoat? Does the seed have germination inhibitors that require leaching? Does the seed have a temperature problem (needing a cycle of cold then heat)? Are they a hybrid seed? Have you looked at the possibility the seed has been deliberately destroyed (gamma radiation at customs or a supplier not wanting you to actually grow the seed)?
As an ex nurseryman, I've seen many of these problems firsthand. One of the most stunning things I have used to bring about germination of viable but stubborn seeds is smokewater. See my profile for a technical paper.
Seed quality is one of the vital factors that decide the performance of seed (percentage of germination, Speed of germination and establishment in field). We have seed testing and seed certification agencies to monitor these seed quality. Quality does not only includes the physical but also the genetic quality (purity) is of pivotal importance.
Yes surely affects. Seed moisture also important. Good seeds you get 95 percent germination and seed with low quality or old seeds less germination percent. Can be improved by seed priming the germination and vigour of seed and uniformity , reduce the number of days for germination.
Published:1. Legume Research -An International Journal: Biochemical Investigation on vigour enhancement in fresh and aged seeds upon seed priming in cowpea. PUblished online Jan 2021.
2.Effects of seed priming on biochemical parameters and seed germination in cowpea [Vigna unguiculata (L.) Walp] . Legume Research, 40 (3) 2017 : 562-570