I would like to stain mouse skin using RNA scope as part of my doctoral thesis. However, the probes are too aggressive and cause the skin to dissolve. Does anyone have experience with this and any valuable tips?
I have performed a fairly standard kinetics experiment, in which I investigated the stability of various oligonucleotides in an enzyme solution, by incubating the oligonucleotides with the enzyme...
17 April 2024 3,655 8 View
Hello! Are there reserved variables in CST, for example, the speed of light, mass and charge of an electron? What value for the speed of light is used in CST?
31 October 2023 8,476 2 View
Hello everyone! We received Influenza B Control Antigens BPL inactivated for HAI. It looks like slightly yellow transparent fluid with white flakes. I can't find any information on IRR website,...
08 August 2023 9,500 0 View
It is known that sample conductivity affects pulse durations in 1H NMR experiments. But how does the concentration of NaCl in a sample affects pulse durations in sodium-23 (23Na) NMR experiments?...
18 June 2023 1,708 4 View
Hello! I would like to address the experts regarding a question about conducting a statistical analysis using only nominal variables. Specifically, I would like to compare the responses of survey...
01 April 2023 3,688 4 View
I want to test whether my anti-HA monoclonal antibodies can inhibit the membrane fusion step of influenza virus entry, but we don't have any resources for syncytium formation assay with...
07 February 2023 2,731 0 View
Please explain what is going on with this platform? Authors send me links to full papers and a url defense pops up. At this site, the papers remain inaccessible. It is impossible to request...
13 December 2022 9,525 0 View
Hello, I have a question about double layer agar method for plaque assay for mycobacterium. Why my bacterial lawn of Mycobacterium smegmatis is not homogeneous? What could be the reason of growth...
02 November 2022 7,925 0 View
Hey everyone! Have anyone faced an issue of how to minimize/quench autofluorescence in mammalian live cells? To be more specific I always stained and visualized ovarian cancer cell lines in blue,...
17 February 2022 2,492 0 View
Hey everyone! Would be really appreciated for advice. Trying to solubilize plasma membrane with Styrene - maleic acid (SMA) copolymers. Following all the instructions making a 5% SMA solution,...
04 August 2021 5,572 1 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View