Does rice root total protein degrade on heat treatment?

21 March 2022 0 8K Report

Hi researchers

I am working specifically on rice root proteins for proteomics. Now I am at the stage of standardization of extraction buffer for rice roots. I followed Raorane, Narciso, and Kohli 2016 (attached below) protocol. According to protocol heat treatment is included and I followed the same. I observed degradation of proteins (image attached). For reference I had even included rice shoot samples.

Gel image

Lane 1- Protein marker

Lane 2 and 3- Root sample with heat treatment

Lane 4 and 5- Shoot sample without heat treatment

Lane 6 and 7- Shoot sample with heat treatment

Can anyone please suggest me any specific working protocol for rice root total protein extraction?

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