It should be okay as long as the safety glass is down, as UV is highly absorbed by glass.
Jiri is right, but if one works long hours under UV exposure, it's better to wear safety glasses, gloves and labcoat. The main concern is related to UV irradiation on human eyes.
What kind of cabinet? For photographing gels? Or an open UV table to cut out gel fragments? The first one should only work when the door is closed, in the second case wear eye (or better face) protection, gloves and long sleeves (lab coat). I never got "sun"-burned when I followed these precautions.
If you're talking about an exhast hood it should be fine if you wear lab coat, gloves and if the glass is down. UV rays can cause the dimerization of thymine bases in DNA but if they are confined and you are in safe conditions you shouldn't have problems.
thank you for Ur suggestions.i will work minimum 1 hr every day.so i will try follow Ur tips and bio safety cabinet 2nd grade.at present i am leaving 10 seconds for air flow after UV off before going to do my experiment.
@Venkatesh: You are talking about cell culture work? Why would you leave on the UV light while working? This is not necessary and also will affect the effectivity (if there is any, I don't like UV in the hood too much anyway) severely. The best results are obtained when the hood is more or less empty, since everything, which is inside casts shadows and areas where no UV light can get. Also many plastics are sensitive to UV light exposure.
yes sir i will do regular microbiological work like sub culturing and anti microbial sensitivity test,bio typing.before using the bio safety cabinet i will on UV 20-30 mnts for sterilization.After that i will on airflow when i am performing.
It depends on the wavelength and the exposure duration. Since you worn lab coats most likely the injuries to the body would be minimal. However, you must have a check up on eyes and any exposed skin areas as you might have issues later
pls ans if in plant tissue culture lab we expose any plant cutting to UV light of LAF what will be the effect?
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