Does pseudomonas aeruginosa form a clumpy cell in tryptic soy broth?

Can I get information about research laboratories that are currently having in culture, internationally/locally standardised microorganisms?

Microorganisms of interest include; E.coli; Salmonella typhimurium; Pseudomonas aeroginosa; Streptoccocus pneumoniae; Staphyloccocus aureus and Candida albicans. Locations closer to Port...

18 February 2021 6,500 3 View

Any natural extract (that is easily available) or any drug combination triple which i can use against gram negative?

I'm working on gram negative i want to use any natural extract (plant extract) or triple drug combination against its biofilm formation.

14 February 2021 1,403 3 View

Question regarding investigation of the nature of bacteria: whether it can cause human diseases or not?

Hi all, Considering that one has the whole genome sequence (annotated) of a bacterium and wants to know if it has the potential to cause human diseases, what steps would you recommend...

26 January 2021 8,732 3 View

Pseudomonas that lose their fluorescence?

I am working on Pseudomonas fluorescent strain identification, I found that some of isolate that I have been conserve at 80c last year have loosing their fluorscent appearance on new culture....

09 November 2020 9,181 4 View

Can anyone tell me the list of antibiotics which are sensetive and resistanc to Pseudomonas flourences and Pseudomonas puituda bacteria ?

I am now working on conjugation process between Pseudomonas flourences and Pseudomonas puituda bacteria, so in order to confirm that the genes are transferred, we need to use antibiotics, so...

31 October 2020 509 3 View

How many ways do bacteria have to communicate?

Hello, I am researching into how bacteria communicate. What I've found is that they use quorum-sensing*, and electrical signals**. I've also come across the ability to "speak and listen"***, but I...

03 September 2020 9,168 8 View

The medium used in LasA staphylolytic assay and LasB Elastin congo red assay?

I'm currently collecting references about staphylolytic assay to detect LasA protease activity and elastin congo red assay to detect LasB elastase activity in Pseudomonas aeruginosa, but from the...

03 August 2020 1,160 3 View

Bacterial siderophore production test/modified CAS assay results interpretation?

I've been working with plant bacteria and investigating their ability to produce siderophores. For a qualitative test I chose a method described hereModified microplate method for rapid and...

26 July 2020 7,194 4 View

NirS qPCR assay underestimating by 2 log?

My lab designed a qPCR assay for amplifying the denitrification gene nirS from DNA extracted from soil. The assay has worked perfectly in the past with almost perfect efficiency and gene copy...

20 July 2020 8,070 1 View

Why are bacteria washed with NaCl solutions or some specific buffer before any photolysis experiment?

People who work with bacteria, I have been looking at literature and every time a photolysis experiment to thermally kill bacteria is done, the bacteria are spun down, the supernatant growth...

14 May 2020 9,071 5 View

Sweta Singh

Pseudomonas may give a pigmented growth but clump formation raises the suspicion of possible contamination. U can do spot microscopy to check that from broth itself

Roger Bayston

Ps aeruginosa strains vary in their phenotypic behaviour regarding smooth/rough, mucoid, etc growth and this strain might be one that shows clumping in fluid media. Replate to check Dr Singh's suggestion of contamination, but if you get pure growth Ps aeruginosa then it is a clump- forming strain. This is often affected by cell hydrophobicity so you could try another medium