Oxford's Nano-pore Sequencing Technology
The MinIon does not use the MSpa protein nanopores. (see also: https://www.dropbox.com/s/bkehpxn6fw8d00o/nanopore.pdf?dl=0). It seems that they use (probably heavily mutated) CsgG nanopore.
Hello, I've recently synthesized a short (17aa) peptide via manual SPPS. I was instructed to submit a small pipette tip's worth of material onto a capillary column to obtain a rough estimate of...
12 January 2021 9,432 3 View
SPPS of a 16 aa peptide was performed on a 0.15 mmol scale. Resin gained a minimal amount of weight: starting weight of 224 mg and a final weight of 280.I believe my peptide failed to elongate but...
21 December 2020 9,428 11 View
Greetings to all. I have some questions about setting up DFT calculations in Gaussian software (I am a beginner in these matters), and trying to calculate the energy of the first excited triplet...
06 December 2020 3,504 4 View
I have an 18aa azido-peptide conjugated at the N-terminus (cysteine). Synthesis and folding were validated through HPLC and mass spec. Multiple attempts were made with Dylight-phosphine conjugates...
02 August 2020 2,775 3 View
Hello, Could anyone here please tell me the experimental set up, or the protocol to be able to measure the angular distribution of the light emitted (fD) by some OLED device? I have seen that...
10 February 2020 7,932 2 View
Hi! I recently installed the Real Statistics complement for building Bland-Altman plots in Excel. However, I don't know how to use it in evaluating the agreement between HRV and PRV series. Could...
25 November 2019 3,736 3 View
The minimum number of RR intervals required for reproduce several metrics derived from HRV (e.g., HF power) can be found in the literature (Richards et al., 2010). However, what about PPG signals...
02 June 2019 353 2 View
Who can help with a link to the a fast publishing scopus indexes journal. I am looking at a journal that will respond in like 3-5 weeks
19 August 2018 1,327 18 View
In bioinformatics databases play an important role in the results, whether chimera detection or OTUs assignment (16S metagenomics). In your experience, which databases have a better relation...
11 January 2018 858 3 View
I am having difficulty meshing the attached geometry for a two phase mixing type application where I care about the volume fraction at various points along the tubes. I have defined 3 bodies Body...
05 December 2017 1,306 3 View
Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...
02 March 2021 4,569 4 View
I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...
02 March 2021 752 2 View
During a computational analysis, I found that for a protein of a plant, two different subcellular localizations are seen using CELLO and WoLF PSORT. For example, in CELLO, it identified the...
01 March 2021 6,729 2 View
I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...
28 February 2021 4,521 3 View
Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...
27 February 2021 1,965 1 View
i am trying to download x'pert high score software, but can't download it. please do suggest software for phase analysis
24 February 2021 5,687 4 View
I want to understand the basics of docking, and also know about all bioinformatics tools which are free of coast and helpfull in in-silico analysis?
22 February 2021 8,879 2 View
I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...
22 February 2021 4,374 3 View
Hi I am running a simulation in Abaqus for a concrete beam. Its fine aggregate replaced with sea-sand. I was wondering if I could find studies that measured the ultimate strain of concrete made...
22 February 2021 6,409 2 View
We are conducting survey research on COVID-19 misinformation among students. How can we validate our survey for it to produce valid and reliable data? Also, what statistical tools can we use to...
16 February 2021 678 3 View
