Recently, I'm investing the wound healing activity of some substances through the scratch assay. The initial intention was to use NIH-3T3 fibroblasts cell line, but I only have 3T3-L1 cells (pre-adipose fibroblasts) available.

It would be possible to perform the migration test using 3T3-L1 and It will have some influence on the results?

Could someone clarify the main differences between these two cell lines and can they respond differently in the migration test (scratch assay)?

Thanks all!

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