Inorganic phosphate increases the rate of tension redevelopment following rapid shortening when the thin filament is fully saturated with Ca2+.
I am wondering if inorganic phosphate also affects the rate of tension rise upon Ca2+ activation of a relaxed skeletal muscle fibre (i.e. one with ADP-Pi in myosin's nucleotide binding pocket and Troponin C not already occupied with Ca2+)? Photo-release of caged Ca2+ or rapid solution changes with myofibrils would seem the most applicable experimental techniques to answer this question. My literature search is coming up empty.
I'm hoping the community can help.
Thanks in advance!
It seems Pi does accelerate the rate of force development upon release of caged Ca2+ (at 5 deg C at least).
Article The Effect of Inorganic Phosphate on Force Generation in Sin...
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