Does incubation of soil sample with polyvinylpolypyrrolidone (PVPP) affects DNA and proteins in soil?

03 January 2015 1 8K Report

Polyvinylpolypyrrolidone (PVPP) is usually used to clean samples from interfering substances (e.g. humic acids). Does incubation of soil sample with 10%(w/w) PVPP affects DNA and proteins (specifically enzymes) in soil?

Samir A. Haddad

PVPP was shown to be ineffective during cell lysis, but efficient when used as a spin column during the nucleic acids purification step.

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