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I am looking forward to analyze changes in soil bacterial community by 16S amplicon sequencing. Previously there were more reports on targeting V1-V2 region with primer sets of 27F/514R(or...
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Polyvinylpolypyrrolidone (PVPP) is usually used to clean samples from interfering substances (e.g. humic acids). Does incubation of soil sample with 10%(w/w) PVPP affects DNA and proteins...
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