Why my RNA is not good?

I am working in a laboratory with Cov19. Extraction is doing kit but it isn't good and the amount RNA, 260/280 is higher than 3. Can someone help us in this regard?

12 September 2020 2,827 7 View

Hello . Is there any stereo video dataset to produce depth ?

I'm going to produce depth video using a video with the right camera and a video with the left camera,(I mean two videos taken with the stereo camera).

16 October 2019 5,014 3 View

Proving the formation of NiCoFe2O4 nanoferrite by XRD?

Hi I synthesized NiCoFe2O4 nanoferrite. I used XRD to prove the formation of NiCoFe2O4 nanoparticles. I understood that the position of the peaks is correct. I mean that the nanoferrite powder...

07 December 2016 7,735 16 View

Hydrogen peroxide effect on vitamin c?

does vitamin C and H2O2 have any chemical reactions which will prevent us from detecting Vitamin C?

15 August 2016 1,715 3 View

I have trouble in detecting my protein of interest in total cancer cell line lysate in western blot. How should I solve this problem?

I can detect beta actin band and my cell line is MDA-MB-231 breast cancer cell line.

29 September 2015 7,619 20 View

How can I find a displacement table from stress-strain curve in order to use it in damage evolution in Abaqus?

I'm working on uniaxial tensile test for an epoxy and crack propagation in it. I have data from my lab work and now I would like to model it in Abaqus. I made the bone shape sample and everything...

03 February 2015 8,650 1 View

What is the difference between the prevalence of insulin resistance found in those with NASH versus those with Hepatitis C?

NAFLD shares many overlapping features with the insulin resistance syndrome, also known as "Syndrome X" including central or visceral obesity, type II diabetes, and dyslipidemia. Of course, NAFLD...

14 September 2014 882 5 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

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02 March 2021 3,060 3 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View