Does hypoxia or re-oxygenation increase reactive oxygen species (ROS) production in intestinal epithelial cells? Has anyone measured ROS production in IPEC-J2 cells during hypoxia or reoxygenation?
The likely scenario is that at different stages of ischemia-reperfusion, different reactive oxygen species rise and fall with distinct kinetics and abundance.
Typically the reperfusion stage gives rise to damaging highly reactive oxygen species (hROS), supposedly including hydroxyl radical.
What's not clear is exactly which of such ROS appear(s), from where (cellular sources), why and how they may in fact interact.
Therefore it is a highly complex problem (containing many sub-problems to be tested and clarified).
See:
http://www.ncbi.nlm.nih.gov/pubmed/8530542
http://www.ncbi.nlm.nih.gov/pubmed/9358241
Theoretically hypoxia can uncouple the mitochondrial oxidative phosphorylation and increase ROS formation. But, I have never read related articles since it is not my field of research. It is even more possible if the cells experience hypoxia and re-oxygenation due to impaired mitochondrial ETC. But, I do not know how long te hypoxia needs to be to have the effect.
It is clear that during hypoxia, due to lack of oxygen, ROS can not be produced. But, with the onset of reperfusion blood renders oxygen to activated cells and they use this oxygen to generate ROS.
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