Does human CD16 bind to rat IgG1s?

06 December 2018 1 8K Report

We are wondering if human NK cells can mediate ADCC against targets labeled with a rat anti-mouse antibody.

Hi Allen,

Looking product below there is cross reactivity between human FcgRIIIb and rats IgG's:

https://www.lsbio.com/targets/fcgr3b-cd16b/g194741?fq=FilterReactConf%3a%22Rat%22,FilterReactConf%3a%22Human%22,FilterTargNameUrl%3a%22FCGR3B+%2f+CD16B%22&prefix=a#SearchTable_div

FcgRIIIb rats and humans are not quite similar but in many positions sequence is the same (see Uniprot alignment: Uniprot 1.JPG), (https://www.uniprot.org/align/A20181231E5A08BB0B2D1C45B0C7BC3B55FD2655602380CP this link works only 7 days).

Affinity of rats IgG's for human CD16b probably will not be so strong like human IgG but looks it is possible.

Best regards,

Tomasz

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