I am trying to extract genomic DNA for pyrosequencing simultaneously with RNA using Qiazol (which I presume is just Trizol from Qiagen). After RNA extraction, I extracted DNA from the phenol/interphase following the manufacturer's instructions [i.e. add 100% ETOH to interphase&phenol phase, centrifuge (2000xg, 2min), remove supernatant, add Na citrate (0.1 M in 10% ETOH) and incubate for 30 min at RT, centrifuge (2000xg, 5 min), remove supernatant and repeat, add 75% ETOH to pellet and incubate for 20 min at RT, centrifuge (2000xg, 5 min), remove ETOH and allow pellet to air dry. Redissolve pellet in 8 mM NaOH (left at 4C overnight)].

After spectrophotometer analysis, my DNA concentrations seem quite low and as are my 260/280 ratios. Can anyone advise how I can improve my DNA yield and purity?

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