I am currently performing siRNA transfection experiments using new liposomal carriers and would like to optimize the siRNA concentration for efficient knockdown with minimal cytotoxicity. I wonder whether the siRNA–liposome complex should be formed directly at the desired final concentration, or whether it is acceptable to dilute it post-complexation to reach the desired concentration. Any insights or references would be greatly appreciated.
Hi!
From a technical perspective, siRNA–liposome complexes should ideally be formed at the final desired siRNA concentration to maintain optimal charge ratios (N/P ratio) and complex stability, which directly influence transfection efficiency and cytotoxicity. Post-complexation dilution can disrupt particle integrity or surface charge, potentially reducing uptake or silencing efficacy. However, minor dilutions (≤2×) in serum-free media may be tolerated without significant loss of function. It is best to optimize complexation conditions empirically and refer to carrier-specific protocols.
- Badges
- Science topic
- Similar topics
- Phytochemistry
- Extracts