Phan Linh

5 Questions 5 Answers 0 Followers

Questions related from Phan Linh

How to use the Liposome Kit L4395 (Sigma)?

Has anyone worked with the Liposome Kit L4395 (Sigma)? I noticed that each kit contains five vials with a relatively large volume. I’m considering aliquoting one vial into smaller tubes for...

10 June 2025 561 6 View

Does the optimal N/P ratio vary depending on the type of cationic amide used in liposomal siRNA delivery systems?

I'm currently optimizing a liposomal siRNA delivery system and considering stearylamine as the cationic lipid. I would like to know whether the N/P ratio needs to be adjusted compared to...

07 June 2025 9,339 3 View

Can I use calcein am/ ethidium homodimer-1 kit to evaluate the viability of sperm?

Hi all, I use calcein am/ ethidium homodimer-1 kit to stain sperm. But in some cells I see both green and red signals. Should I evaluate those cells as live or dead cell? Thanks all

26 June 2023 6,254 0 View

How to prepare agarose beads for SEM analysis?

Hello everyone, I need to analysis the morphology of my agarose bead by SEM. Should I fix the sample with glutaraldehyde or something similar before drying? Which procedure is suitable for agarose...

06 March 2023 6,444 2 View

Does forming siRNA–liposome complexes at the final working concentration improve transfection efficiency compared to post-complexation dilution?

I am currently performing siRNA transfection experiments using new liposomal carriers and would like to optimize the siRNA concentration for efficient knockdown with minimal cytotoxicity. I wonder...

01 January 1970 7,057 1 View