Can we pattern graphene using UV light ?

03 April 2017 3,541 6 View

How to perform cyclic voltammetry using the 3-electrode setup in 0 V to -0.8 V range in basic electrolyte?

I have to check CV characteristic of my pseudocapacitor electrode which was made using reduced graphene oxide and ZnO nano particles. I am using Ag/AgCl as reference electrode, Pt foil as a...

01 January 1970 6,294 5 View

How can we calculate the dielectric constant of electrical double layer if known the ionic concentration at the electrode?

For two electrode system, through simulation if I know the concentration of ions (K+ and OH-) at the electrode, is it possible to calculate dielectric constant of the EDL? Thank You.

01 January 1970 7,448 2 View

Why polytetrafluoroethylene (PTFE) is hydrophobic in nature?

Since PTFE has flouride atoms on it backbone, therefore due to the electronegativity of floruride atoms, it should interact with water molecules by forming hydrogen bonds making it hydrophilic,...

01 January 1970 5,929 7 View

Is it important for a reference electrode to be in contact with the solution while performing cyclic voltammtery using 3-electrode system?

Trying to understand the significance of reference electrode interaction with the solution in which it is submerged (usual practice) with other electrodes (working and counter). For exp. - For...

01 January 1970 2,335 8 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

How can I edit suggested publications?

Hi there! I regularly receive suggestion about papers and authors that are totally out of my area. Does anybody know how I can fix this? Thank you!

01 March 2021 9,574 4 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View