What is the biggest weakness of anti slapp regulated especially in environmental aspect??

many countries that have regulated of anti-slapp as an effort to protect the rights of public participation. unfortunately they still have many weaknesses in its implementation.

21 March 2019 9,900 3 View

Please help, Why does fiber-optic pressure transducer get very variable and highly improbable readings after being submerged in water?

We recently bought a fiber-optic pressure transducer from Opsens (the machine is called Lifesens). We ar einterested on using it to measure intracranial pressure in vivo. However, we had just...

13 May 2018 6,215 2 View

How can I avoid fibroblasts growth in a cell culture of bladder cancer from mouse?

I am trying to establish cell cultures from a sample of bladder cancer that we develop in mice but we have many fibroblasts in our cultures. Does anyone knows how to inhibit them without...

18 June 2017 9,142 4 View

What is the effect of room temperature on brain tissue intended for single nuclei RNA sequencing?

I am planning to do single-nuclei sequencing for brain organoids. However, the organoids experienced 25 minutes in room temperature prior to freezing. Most of the time they were in their culture...

17 January 2021 5,257 1 View

Why are the organoid cells showing blue fluorescence even without dapi stain?

Mouse intestinal organoids ; After fixation and storage in PBS-T, when I checked in microscope, the organoid show fluorescent color blue even if I have not added DAPI. Does anyone have such...

05 January 2021 2,067 3 View

How do you fix organoids for IHC staining and H&E sections??

I work with ovarian cancer cell line organoids embedded in matrigel, they grow well the A2780 cells, but once I start using 10% formaldehyde to fix them they disintegrate...does anybody have a...

29 November 2020 1,900 1 View

What is the best way to get organoids of the same size?

I would like to get organoids of the same size. We filter organoids using a 100 um filter and then pass that filtrate through a 40 um filter followed by flipping the 40 um filter. Then we wash the...

22 November 2020 9,391 3 View

Is this yeast contamination and how do I get rid of it?

I received this gastric tumor biopsis and processed using standard protocol to establish gastric cancer organoids. Contamination looks like this. I have never seen this before and the culture...

08 November 2020 8,541 4 View

How to successfully produce agarose microwells with PDMS stamp?

05 October 2020 3,696 3 View

Insight on Unusual Organoids and Contamination?

In the past few weeks I've run into some familiar and unfamiliar problems with my mouse small intestine organoids grown in IntestiCult media. I've attached photos and I'm wondering if anyone has...

11 August 2020 5,838 3 View

Is it possible to create an organoid containing stroma?

Hi, I work on mouse oviduct organoids and would like to study the stromal cells. Is it possible to create an organoid containing stroma? So far I have created organoids from mouse oviduct tissue...

04 August 2020 9,236 1 View

Can anyone suggest what are these large bubble like structure along with small organoids and how to get rid of them?see the attached image.?

I am growing 3D organoids from mouse lung AT2 cells in matrigel. I am using glutamine, Penstrep. 10% FBS, ITS and tGF b inhibitor in media.after 6 days I observe large bubble like structure in the...

02 January 2020 1,352 5 View

How do you induce murine intestinal enterospheres to develop into true organoids?

We extracted murine intestinal epithelial organoids using the StemCell Technologies protocol. We had a good number of organoids develop. I then passaged these organoids, and apparently lost them...

08 September 2019 1,521 1 View