Does Escherichia coli DH5 alpha strain contain fertility plasmids? What is the parent strain?

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Hanna Kok

https://www.thermofisher.com/order/catalog/product/18265017

F' Episome (to make ssDNA): Lacks F' Episome

So no, it doesn't contain fertility plasmids

Hafid Soualhine

Its a F- strain. its parent strain is DH1 and both derived fron MG1655 strain.

The original strain was described by Meselson , Nature 1968 (PMID 4868368).

DH5alpha was turned to DH10Beta and commercialized as TOP10 By invitrogen.

The original strain was cured of Lamda phage by UV light and then cured of F plasmid by acridine orange

Here a list of E. coli strains used

http://openwetware.org/wiki/E._coli_genotypes#DH5.CE.B1

Syed A Ali

Dear Hafid, I highly appreciate your informative post.

Jaroslaw Krol