Eniyan Kandasamy

4 Questions 6 Answers 0 Followers

Questions related from Eniyan Kandasamy

Can CTAB solution turns into a gel at room temperature?

I prepared 10% CTAB in 0.7M NaCl and at room temperature it is still in liquid form but l learnt from colleagues that it forms a gel at room temperature. Will it impact the yield in DNA...

07 July 2018 1,461 1 View

Can a single aminoacid substitution renders protein insoluble?

Mutated a Arginine residue with Alanine by SDM in the active site and the protein became insoluble. WT protein was found soluble but the mutated one was found in pellet fraction.

07 July 2016 3,111 4 View

Does Escherichia coli DH5 alpha strain contain fertility plasmids? What is the parent strain?

what is the parent strain for Escherichia Coli DH5alpha and does it have F plasmid?

01 January 2016 5,899 4 View

Do we need to add 0.1% SDS in Transfer buffer for transferring a 97kDa protein?

I did western blotting for a 55kDa and 97kDa protein and used 1:5000 dilution of Primary antibodies and 1:10000 dilution of secondary antibodies. The blot was developed using DAB and i got a good...

04 April 2015 6,806 7 View