The 1st link nature.com deals with amplifying V3 region then DGGE with 25%-65% urea and formamide.
The second link deals with V6,V7 and V8 and gradient at 40%-60%.
Since my proposal centers on using mice gut microbiota I thought it would be appopriate to use the gradient at the 1st link. While using V6,V7, and V8 regions would be helpful to me since I already know the forward and reverse primers used (primers - 3rd link). The 1st link did not write the primer sequence.
http://www.nature.com/srep/2014/140801/srep05922/full/srep05922.html#methods
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC126735/
http://www.philsciletters.org/pdf/2011n2.15.pdf