I did a staining on retina explant culture slides with ABC/DAB for PAR. In order to see the delimitation of ONL and INL better I also used vectorshield with DAPI for counterstaining. I took some pictures of the stained slides before vacation and was able to see DAB/PAR staining but after the holidays i retook pictures with the same slides and the PAR DAB staining wasn´t there anymore.
In theory, DAB should be stable for years in my slides and it is odd that it wasn´t there after a 3-week holiday. Does someone have experience with this and could tell me if the DAPI could have had any effect on this?
Hello Alexander, did you made another try with your samples? I'm interested in the same protocol, but I'm worried about the same issue.
Do you have new information about the interaction between the two technics?
Thanks a lot for your answer
Hello Mario, sorry for answering so late.
Our conclusion was that DAPI does not interfere with DAB staining. The main problem is if you store your slides for longer time (2 weeks or more), then it is a big problem to visualize any DAB staining.
So all in all, you can use both together, but take the images as fast as possible.
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