Hi

all contaminant DNA can affect molecular biology protocols as hybridization, amplification....It is therefore recommended to run DNAse I cleaning before using your samples. Nanodrop will only give you some statistics and can be confusing, the best is gel electrophoresis or Agilent bioanalyzer if you get one.

all the best

fred

Yes, contaminant DNA significantly affects the investigation of m6RNA, particularly when using antibody-based sequencing methods, leading to false-positive signals and inaccurate quantification. The extent of the effect depends on the level of contamination and the specific analytical techniques used.

Yes, you should follow the instructions in the kit if you want it to work.

A nanodrop (or other spec) will tell you the concentration of nucleotides in the solution. They will not differentiate between DNA and RNA and will not tell you anything about the length of the molecules (intact vs. degraded). An agarose gel will show you if the RNA molecules are intact vs. degraded.

You should chat with someone who does this protocol since it sounds like you are very new