Does components of MRS media can hinder in colony PCR?

12 December 2024 0 6K Report

I am using MRS media (for lactobacillus growth), containing peptone, yeast extract, meat extract, glucose, potassium phosphate, sodium acetate, magnesium sulfate, Manganese Sulfate, Tween 80 and ammonium citrate. I am performing colony PCR but no results appeared. So, is there any hindrance due to media components?

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