I've had a lot of problems recently with bacterial contamination in my long term cultures (NK and MM cell lines). I've got Cipro and I know it's effective at killing the bacteria, but is it a bad idea to add it to some of my cultures as a preventative measure? Will it just destroy the healthy cells?
Please review the following
USE OF CIPROFLOXACIN AND BM-CYCLIN IN MYCOPLASMA DECONTAMINATION
In Vitro Cell. Dev. Biol. 28A:708-710, November-December 1992
In conclusion, ciprofloxacin proved to be a better choice of antibiotic in the mycoplasma eradication program of our contaminated cell lines because of its simplicity in use, effectiveness in the elimination of mycoplasmas, lack of re-emergence of infection and its lack of cytotoxicity. In contrast, we found BM-Cyclin to be very cytotoxic to all the 9 cell lines examined in this study. Furthermore, we found the sequential and cyclic use of 2 different antibiotic combinations in BM-Cyclin and the necessity to empirically determine the optimal concentrations of these 2 antibiotics for treating BM-Cyclin sensitive cell lines both time-consuming and cumbersome.
We are also facing the same problem in our cell culture. Use of cipro is inducing damage to the genomic DNA but it is reversible, which i observed after doing H2AX foci forming assays. Therefore it is not good to use cipro for long term.
Dear Ashley, we successfully used ciprofloxacin in primary cultures of mouse lymphocytes and did not see any significant difference in T cell functions if compare with standard antibiotic mixtures. Very good, non-toxic and reliable reagent to avoid bacterial growth.
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