I want to transfect phage T2 genome into HEK293 cells in order to produce phage particles and cell lysis. But I don't know whether the genes will work or not. What is phage biology in mammalian cells?
My challenge is that may a phage gene expresses in mammalian cell?
@Saleha, @Charlotte: Thank you
06 July 2016 7,800 12 View
I know Bcr-Abl fusion gene that is used as an cytogenetics marker for ALL. Thank You...
06 July 2013 3,554 11 View
Circular DNA makes a smear when running in agarose gel, but the digested linear form doesn't. I am not talking about covalently or supercoiled bonded.
06 July 2013 7,438 11 View
These are AGS cells (Gastric cancer cell line) after incubation in a low pH stale RPMI + 1% L-glutamine +10% FBS + 2% antibiotic for 10 days without medium refresh. When I check the cells most...
05 June 2013 5,336 8 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...
03 March 2021 8,796 3 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
03 March 2021 5,360 2 View
02 March 2021 3,060 3 View