My colleagues and I have designed a survey to compile data about fish gonad or eggs rRNA profiles. Recently a series of transcripts related to the process of have been described as useful markers for the molecular identification of sex and of xenoestrogenic effects in teleost fish. An electrophoresis of total RNA extracted from gonads allows identifying the presence of oocytes due to the high 5SrRNA expression levels (Diaz de Cerio et al, 2012). This technique has been probed also in some commercially relevant fish (Rojo Bartolome et al, 2012a,b).

So now… Researcher, we need your help!

Basically, we want to create collaborative network to extend developed 5S/18S rRNA index as a robust method to identify fish sex and female reproductive stage in fish with different reproductive strategies and in different environmental scenarios. And to build a database for fish gonad/egg rRNA profiles.

For this purpose, we need Bioanalyzer 2100 XDA format files of fish gonad/egg samples (and related histology pictures if it is possible).

Tutorial at: https://youtu.be/Tpd_v5NY-pE

Your benefit:

All data generated from your samples will be made available to you. And if sufficient information were gathered we propose to write a revision paper in which a member from each contributing laboratory will be invited to sign.

Interesting Reading

Diaz de Cerio O., Rojo-Bartolomé I, Bizarro C, Ortiz-Zarragoitia M , and Cancio I. 2012. 5S rRNA and Accompanying Proteins in Gonads: Powerful Markers to Identify Sex and Reproductive Endocrine Disruption in Fish. Environ. Sci. Technol., 2012, 46 (14), pp 7763–7771.

Rojo-Bartolomé I, Diaz de Cerio O, Cancio I. 2012. 5S rRNA in gonads, a powerful marker for the identification of sex in fish. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology. 163. S55.

Rojo-Bartolomé I, O. Diaz de Cerio, J. Martos-Bernal, E. Bilbao, M. Ortiz-Zarragoitia, I. Cancio. 2012. 5S to 18S rRNA ratio in gonads as an easy and inexpensive index for the identification of sex in a pollution sentinel fish species (Chelon labrosus). Comparative Biochemistry and Physiology, Part A 163 (2012) S55–S60.

https://youtu.be/Tpd_v5NY-pE